human bone plates Search Results


osteoassay tm human bone plate  (Corning Life Sciences)
90
Corning Life Sciences osteoassay tm human bone plate
(A) RNAs from RAW 264.7 cells cultured with normal culture media (C), sRANKL (R; 100 ng/ml), TNF-α (T; 10 ng/ml), or 50% human GMC conditioned medium (sup.) were extracted after incubation for 5 days. The isolated RNAs were then reverse transcribed, and five osteoclast marker gene transcripts (ATP6v0d2, c-src, DC-STAMP, integrin β3, and vATPase) were detected by real-time RT-PCR. GAPDH was used as internal control. Fold increase from control in each transcript is shown. All experiments were performed in triplicate wells for each group. Results are expressed as mean ± SD. * p<0.001 vs. control. RAW 264.7 cells were co-cultured on <t>OsteoAssay</t> plate (Fig. 6B) or Osteologic plate (Fig. 6C) with 50% human GMC conditioned medium harvested from GMC culture with or without anti-TACE antibody. In some conditions, anti-RANKL antibody was added to block sRANKL activity (Fig. 6C). Three human GMC supernatants were analyzed. C: cultured with normal culture media; R: cultured with sRANKL (100 ng/ml). All experiments were performed in triplicate wells for each group. Results are expressed as mean ± SD. *: p<0.05 vs. control. †: p<0.05 between groups.
Osteoassay Tm Human Bone Plate, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/osteoassay tm human bone plate/product/Corning Life Sciences
Average 90 stars, based on 1 article reviews
osteoassay tm human bone plate - by Bioz Stars, 2026-06
90/100 stars
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human bone plates  (Lonza)
90
Lonza human bone plates
(A) RNAs from RAW 264.7 cells cultured with normal culture media (C), sRANKL (R; 100 ng/ml), TNF-α (T; 10 ng/ml), or 50% human GMC conditioned medium (sup.) were extracted after incubation for 5 days. The isolated RNAs were then reverse transcribed, and five osteoclast marker gene transcripts (ATP6v0d2, c-src, DC-STAMP, integrin β3, and vATPase) were detected by real-time RT-PCR. GAPDH was used as internal control. Fold increase from control in each transcript is shown. All experiments were performed in triplicate wells for each group. Results are expressed as mean ± SD. * p<0.001 vs. control. RAW 264.7 cells were co-cultured on <t>OsteoAssay</t> plate (Fig. 6B) or Osteologic plate (Fig. 6C) with 50% human GMC conditioned medium harvested from GMC culture with or without anti-TACE antibody. In some conditions, anti-RANKL antibody was added to block sRANKL activity (Fig. 6C). Three human GMC supernatants were analyzed. C: cultured with normal culture media; R: cultured with sRANKL (100 ng/ml). All experiments were performed in triplicate wells for each group. Results are expressed as mean ± SD. *: p<0.05 vs. control. †: p<0.05 between groups.
Human Bone Plates, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human bone plates/product/Lonza
Average 90 stars, based on 1 article reviews
human bone plates - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
human bone panel i capture plate  (Meso Scale Diagnostics LLC)
90
Meso Scale Diagnostics LLC human bone panel i capture plate
(A) RNAs from RAW 264.7 cells cultured with normal culture media (C), sRANKL (R; 100 ng/ml), TNF-α (T; 10 ng/ml), or 50% human GMC conditioned medium (sup.) were extracted after incubation for 5 days. The isolated RNAs were then reverse transcribed, and five osteoclast marker gene transcripts (ATP6v0d2, c-src, DC-STAMP, integrin β3, and vATPase) were detected by real-time RT-PCR. GAPDH was used as internal control. Fold increase from control in each transcript is shown. All experiments were performed in triplicate wells for each group. Results are expressed as mean ± SD. * p<0.001 vs. control. RAW 264.7 cells were co-cultured on <t>OsteoAssay</t> plate (Fig. 6B) or Osteologic plate (Fig. 6C) with 50% human GMC conditioned medium harvested from GMC culture with or without anti-TACE antibody. In some conditions, anti-RANKL antibody was added to block sRANKL activity (Fig. 6C). Three human GMC supernatants were analyzed. C: cultured with normal culture media; R: cultured with sRANKL (100 ng/ml). All experiments were performed in triplicate wells for each group. Results are expressed as mean ± SD. *: p<0.05 vs. control. †: p<0.05 between groups.
Human Bone Panel I Capture Plate, supplied by Meso Scale Diagnostics LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human bone panel i capture plate/product/Meso Scale Diagnostics LLC
Average 90 stars, based on 1 article reviews
human bone panel i capture plate - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
osteoassaytm human bone plate  (Lonza)
N/A
OsteoAssayTM Human Bone Plate provides a thin layer of adherent human bone for the culture of primary human or non-human osteoclasts, osteoclast precursors, or immortalized cell lines.
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Image Search Results


(A) RNAs from RAW 264.7 cells cultured with normal culture media (C), sRANKL (R; 100 ng/ml), TNF-α (T; 10 ng/ml), or 50% human GMC conditioned medium (sup.) were extracted after incubation for 5 days. The isolated RNAs were then reverse transcribed, and five osteoclast marker gene transcripts (ATP6v0d2, c-src, DC-STAMP, integrin β3, and vATPase) were detected by real-time RT-PCR. GAPDH was used as internal control. Fold increase from control in each transcript is shown. All experiments were performed in triplicate wells for each group. Results are expressed as mean ± SD. * p<0.001 vs. control. RAW 264.7 cells were co-cultured on OsteoAssay plate (Fig. 6B) or Osteologic plate (Fig. 6C) with 50% human GMC conditioned medium harvested from GMC culture with or without anti-TACE antibody. In some conditions, anti-RANKL antibody was added to block sRANKL activity (Fig. 6C). Three human GMC supernatants were analyzed. C: cultured with normal culture media; R: cultured with sRANKL (100 ng/ml). All experiments were performed in triplicate wells for each group. Results are expressed as mean ± SD. *: p<0.05 vs. control. †: p<0.05 between groups.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Soluble RANKL cleaved from activated lymphocytes by TNF-α converting enzyme (TACE) contributes to osteoclastogenesis in periodontitis

doi: 10.4049/jimmunol.1601114

Figure Lengend Snippet: (A) RNAs from RAW 264.7 cells cultured with normal culture media (C), sRANKL (R; 100 ng/ml), TNF-α (T; 10 ng/ml), or 50% human GMC conditioned medium (sup.) were extracted after incubation for 5 days. The isolated RNAs were then reverse transcribed, and five osteoclast marker gene transcripts (ATP6v0d2, c-src, DC-STAMP, integrin β3, and vATPase) were detected by real-time RT-PCR. GAPDH was used as internal control. Fold increase from control in each transcript is shown. All experiments were performed in triplicate wells for each group. Results are expressed as mean ± SD. * p<0.001 vs. control. RAW 264.7 cells were co-cultured on OsteoAssay plate (Fig. 6B) or Osteologic plate (Fig. 6C) with 50% human GMC conditioned medium harvested from GMC culture with or without anti-TACE antibody. In some conditions, anti-RANKL antibody was added to block sRANKL activity (Fig. 6C). Three human GMC supernatants were analyzed. C: cultured with normal culture media; R: cultured with sRANKL (100 ng/ml). All experiments were performed in triplicate wells for each group. Results are expressed as mean ± SD. *: p<0.05 vs. control. †: p<0.05 between groups.

Article Snippet: Pit formation assays To evaluate resorption activity of TRAP-positive multinucleated cells differentiated from RAW 264.7 cells, a resorption assay was performed using OsteoAssay TM Human Bone Plate (Corning, Corning, NY) or osteologic discs (BD Biosciences).

Techniques: Cell Culture, Incubation, Isolation, Reverse Transcription, Marker, Quantitative RT-PCR, Control, Blocking Assay, Activity Assay